A Project Outline

June 24, 2015

Dr. Amer shared a folder with me on Dropbox this morning with a lot of papers and reading. I look forward to getting through them and getting a better understanding on the back ground of this research. He also provided an outline of what I will be doing here.

Cereal Nematodes:

Cyst nematode (Heterodera species)

  • Extraction from soil
  • Counting and collecting cysts
  • How to enhance hatching
  • Inoculation
  • Harvesting

Root lesion nematodes (Pratylenchus spp):

  • Culture preparation
  • Extraction from both soil and roots
  • Counting under microscope
  • inoculation

Crown rot (Fusarium spp):

  • Observing the spores under the microscope
  • How the inoculum is prepared
  • Inoculation
  • Plant harvesting, symptoms and scoring

Visit other programs

The International Winter Wheat Improvement Program (IWWIP) and learn about breeding, rust screening etc.

Visit the quality lab and see what traits they are studying in the quality lab

Help Joe in his work to understand some molecular work especially DNA extraction from plants”

As soon as I got to work I got a call from Gul Abla, explaining my work for the day. She is currently on antibiotics and finally took a day off. We are all hoping she gets better soon.

At lab I spent the morning washing soil samples to get cereal cyst nematodes.

Procedure for washing the soil:

1) Place soil sample in a large pot

2) Fill the pot with water and stir the contents

3) Let the mixture settle for 20 seconds

4) Pour the top water into a sieve apparatus (large one on top and finer one underneath)

5) Repeat the washing procedure for the same soil sample five times

6) Dump the contents of the top sieve and the pot and collect what is in the bottom sieve

In the afternoon I collected the cysts. What I collected from the bottom sieve was a mixture of small rocks, soil particles, and cysts so I had to loo under a microscope to pick out each individual cyst. Each cyst contains hundreds of nematode eggs so later on the eggs will hatch and the nematodes will be used to inoculate new plants. This is used to test whether or not the plant is resistant, tolerant, or susceptible to the cereal cyst nematodes.

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Once I came back from lab I got a chance to read through a little bit of the material Dr. Amer shared with me and it is very helpful!

Around seven I went to the kitchen to cut some fruit for a snack and ran into Cennet Abla. She was making Sikma which is a large tortilla filled with a cheese mixture. I watched for a while in fascination and of course had to try it. It was scrumptious. I don’t know what it is about her cooking, but she adds the right amount of love and skill to make each dish delicious.

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2 thoughts on “A Project Outline”

    1. No, once the cysts are collected we store them in at 4 degrees Celsius till we are ready to use the juveniles for inoculation. Then we rotate between room temperature and 4 degrees to quicken the hatching process. After they have hatched they migrate towards the water in a sieve apparatus. We count the juveniles in 1mL of water and concentrate or dilute it till there are around 300 (the damage threshold). Then we add 1mL to each plant for inoculation.

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